Description
Aviva Systems Biology Elafin/PI3 ELISA Kit (Human) (OKBB00497) is based on standard sandwich enzyme-linked immune-sorbent assay technology. A mouse monoclonal antibody specific for Elafin has been pre-coated onto 96-wellplate (12 x 8 Well Strips). Standards (NSO; A23-Q117) and test samples are added to the wells and incubated. After washing, abiotinylated polyclonal goat detector antibody specific for Elafin is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is visualized through the addition of TMB substrate which is catalyzed by HRP to produce a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Human Elafin captured in well